The AmpliCell slide consists of AmpliGrid, a glass slide for 1ul PCR reactions, plus a removable upper structure that sits tightly sealed on top of the slide and separates 12 growth chambers. Choose from 3 surface coatings (fibronectin, collagen, poly-L-lysine) for optimal growth conditions. After cell expansion, remove the upper chamber structure, wash off excess media, and run up to 4 PCR reactions per chamber.
AmpliCell
Cell Culture-to-PCR on one slide
- On-slide cell culture of adherent cells and cells in solution
- No cell preparation needed => minimized cellular stress
- in situ cell experiments to PCR in few simple steps
- Full visual control of workflow
- 4 PCR replicates per cell culture well
- Save up to 90 % reagent costs
- Compatible to HT systems (e.g., flow cytometry, robotics)
Optical Properties
Ever tried to detect cells in the opaque 3D structure of a microplate? AmpliCell greatly simplifies cell detection because cells are neatly arranged in a monolyer on top of a high quality and ultra-low fluorescence glass substrate. The high optical quality of the AmpliCell slide allows superior control of cell seeding, cell growth and assay success prior to PCR by standard and fluorescent microscopy or image acquisition using fluorescent scanners.
What you see is what you amplify!
Sensitive PCR without sample preparation
After cell expansion, remove the upper chamber structure, wash off excess cells, and run up to 4 independent (RT)-PCR reactions and optional subsequent qPCR on the cells in each chamber. Amplification takes place in 1 µl master mix volumes on each reaction site. Reactions are covered with 5 µl of sealing solution (delivered with the AmpliCell) to prevent evaporation during thermal cycling. The perfect solution to thermo cycle or incubate AmpliCell slides is the AmpliSpeed slide cycler.
To see the convenient workflow please click here: More
