AmpliGrid AG480F
- Single Cell Analysis
- Maximum sensitivity
- Precise optical control
- Ease of use
- DNA/RNA-free
Brochures / Flyers
| Download | File / Size | |
|---|---|---|
| AmpliGrid System: Single Cell PCR – Highest sensitivity down to ONE single cell | pdf / 624 kb | Start Download |
| More insight to the single cell genome. AmpliGrid Single Cell PCR System | pdf / 441 kb | Start Download |
AmpliGrid Automated Cell Detection | pdf / 2.41 MB | Start Download |
AmpliGrid AG480E: Activated microliter reaction slide | pdf / 1.85 MB | Start Download |
Manual
| Download | File / Size | |
|---|---|---|
AG480F / E 1µL Assays User Guide | pdf / 1.59 MB | Start Download |
AmpliGrid AG480F | pdf / 417 kb | Start Download |
How to handle the AmpliGrid System | pdf / 94 kb | Start Download |
Quick Start Guide Beckman Coulter MoFlo™ with AmpliGrid System User Guide | pdf / 267 kb | Start Download |
QuickStart Guide BD FACSVantage™ with AmpliGrid System User Guide | pdf / 324 kb | Start Download |
Quick Start Guide BD FACSAria™ User Guide | pdf / 202 kb | Start Download |
Application Reports
Single cell handling
deposition, optical control, DNA extraction
| Download | File / Size | |
|---|---|---|
Cell sample storage for gene expression analysis on AmpliGrid AG480F | pdf / 484 kb | Start Download |
Visual control of correct cell placement on AmpliGrid slides prior to molecular analysis | pdf / 317 kb | Start Download |
Enhanced DNA amplification from single cells using the Beckman Coulter Cell Extraction Kit | pdf / 344 kb | Start Download |
Gene expression
RT-qPCR, GeXP, miRNA, microarray
| Download | File / Size | |
|---|---|---|
Superior sensitivity in RT-PCR of single cells using the AmpliGrid system | pdf / 371 kb | Start Download |
Quantitative RT-PCR from one single cell using the AmpliGrid technology | pdf / 477 kb | Start Download |
Single cell miRCURY LNA™ assay | pdf / 532 kb | Start Download |
Quantitative multiplex TaqMan® qPCR from single cells using the AmpliGrid platform | pdf / 494 kb | Start Download |
Template amplification and detection of PCR products on AmpliGrid AG480E using microarray technology | pdf / 382 kb | Start Download |
Immunology
virology, bacteriology, infectious desease, cancer biology
| Download | File / Size | |
|---|---|---|
Identifying virus copies in single cells | pdf / 478 kb | Start Download |
High efficiency one-step lysis and PCR from single bacteria using the AmpliGrid System | pdf / 379 kb | Start Download |
Case study: Studying the Viral/Bacterial Connection, One Cell at a Time | pdf / 302 kb | Start Download |
Novel approach for identifying memory B cells during immune responses | pdf / 3.15 MB | Start Download |
Chimera Analysis — improving research on stem cell transplantation | pdf / 1.00 MB | Start Download |
Genetic profiling / Forensics
STR profiling, sequencing
| Download | File / Size | |
|---|---|---|
High sensitivity and cost effective STR profiling on AmpliGrid AG480F using limited sperm material | pdf / 351 kb | Start Download |
Getting full profiles from highly redundant low-volume amplifications (LV-PCR) | pdf / 1.62 MB | Start Download |
Case study: The Evidence Points to Single Cell Analysis for Forensic Applications | pdf / 682 kb | Start Download |
Efficient DNA amplification and cycle sequencing from single cells without template preparation | pdf / 792 kb | Start Download |
Automation
| Download | File / Size | |
|---|---|---|
Contamination-free ultra low volume pipetting on AmpliGrid AG480F slides | pdf / 455 kb | Start Download |
Posters
| Download | File / Size | |
|---|---|---|
Single Circulating Tumour Cell molecular characterization: a feasibility study | pdf / 1.64 MB | Start Download |
Population- and single cell-based strategies to understanding humoral immunity | pdf / 584 kb | Start Download |
AmpliCell and AmpliGrid: A new cell culture and PCR platform for analysis of tumor stem cells | pdf / 3.56 MB | Start Download |
A Reproducible High Throughput Methylome Analysis of a Single Cell | pdf / 818 kb | Start Download |
Zellvergnügen Workflow: Single Cell Molekular Analysis | pdf / 579 kb | Start Download |
Novel approach for identifying memory B cells during immune responses | pdf / 237 kb | Start Download |
Detection of microRNA genes in individual cancer stem cells from breast cancer cell lines using the Beckman Coulter AmpliGridâ„¢ single cell platform and a single cell quantitative RT-PCR method | pdf / 170 kb | Start Download |
Invasionsfreie Pränataldiagnostik auf Einzelzellbasis | pdf / 390 kb | Start Download |
Single cell typing by on-chip Low Volume PCR (LV-PCR) | pdf / 190 kb | Start Download |
Genome-wide expression analysis of single cells using Affymetrix GeneChips | pdf / 1.00 MB | Start Download |
AmpliCell: cell culture-to-PCR based on the AmpliGrid technology | pdf / 595 kb | Start Download |
Scientific Articles
Extended PCR conditions to reduce drop-out frequencies in low template STR typing including unequal mixtures
Forensic Science International: Genetics (2011): doi: 10.1016/j.fsigen.2011.03.002
Human B1 cells in umbilical cord and adult peripheral blood express the novel phenotype CD20+CD27+CD43+CD70-
J. Exp. Med., 208(1), (2011): DOI/10.1084/jem.20101499
Profile of a Serial Killer: Cellular and Molecular Approaches to Study Individual Cytotoxic T-Cells following Therapeutic Vaccination
Journal of Biomedicine and Biotechnology, 2011 (2011): DOI:10.1155/2011/452606):
ERK-Regulated Double Cortin-Like Kinase (DCLK)-Short Phosphorylation and Nuclear Translocation Stimulate POMC Gene Expression in Endocrine Melanotrope Cells
Endocrinology (2011): doi:10.1210/en.2011-0067
A high-throughput DNA methylation analysis of a single cell
Nucleic Acids Research (2011), 1–9, doi:10.1093/nar/gkq1357
PCR on slide: advantages in environmental and clinical microbiology
Applied Microbiology and Microbial Biotechnology (2010)
A simple and innovative method for species identification of phytoplankton cell on minute quantities of DNA
Environmental Microbiology Reports (2010): doi 10.1111/j.1758-2229.2010.00164.x
Circulating tumour cells in clinical practice: Methods of detection and possible characterization
Methods (2010), 50(4): pp. 289-297
Single Cell Analysis of Mutations in the APC Gene
Fetal Diagn Ther (2009), 26(3): pp. 148-56
Technical note: PCR analysis of minimum target amount of ancient DNA
American Journal of Physical Anthropology, 142(2), (2010): DOI:10.1002/ajpa.21268
Miniaturization of biological assays — Overview on microwell devices for single-cell analyses
Biochimica et Biophysica Acta (BBA) - General Subjects, 1810(3), (2010): DOI:10.1016/j.bbagen.2010.04.009
Reduced-volume and low-volume typing of Y-chromosomal SNPs to obtain Finnish Y-chromosomal compound haplotypes
Int J Legal Med (2009), 123(5): pp. 413-418
Preliminary trials of low volume AmpFlSTR® Profiler Plus® amplification using AmpliGrid (AG480F) slides
Forensic Science International (2009), 2(1): pp. 117-118
The combination of single cell micromanipulation with LV-PCR system and its application in forensic science
Forensic Science International (2009), 2(1): pp. 516-517
Automatic retrieval of single microchimeric cells and verification of identity by on-chip multiplex PCR
Journal of cellular and molecular medicine (2009), 10.1111/j.1582-4934.2009.00784.x
Knockdown of transactive response DNA-binding protein (TDP-43) downregulates histone deacetylase 6
EMBO Journal (2009), doi:10.1038, emboj.2009.324
Adult BM generates CD5+ B1 cells containing abundant N-region additions
Eur J Immunol. (2009), 39(9): 2383-94
Single particle adsorbing transfer system
Springer Science + Business Media, Biomed Microdevices (2009) 11: pp. 609–614
Regulation of astrocyte inflammatory responses by the Parkinson’s disease-associated gene DJ-1
FASEB Journal (2009), Vol. 23, article fj.08-125153
Turning to Lipids and Protein
Science Magazine - LIFE SCIENCE TECHNOLOGIES (2009)
Multiplex rt-PCR expression analysis of developmentally important genes in individual mouse preimplantation embryos and blastomeres
Biol Reprod. (2009), 80(1), pp.194-202
Bringing Laser Microdissection and on-chip PCR together
Profiles in DNA (2008)
Single lymphocytes from two healthy individuals with mitochondrial point heteroplasmy are mainly homoplasmic
Int J Legal Med. (2008), 122(3), pp.189-97
Low volume amplification and sequencing of mitochondrial DNA on a chemically structured chip
Int J Legal Med. (2007), 121(1), pp.68-73
Low-volume amplification on chemically structured chips using the PowerPlex16 DNA amplification kit
Int J Legal Med. (2006), 120(1), pp. 42-48
Protocols
Cell deposition
quick start guides, slide adapters
- PALM Adaptor
- QuickStart Guide MoFlo
- QuickStart Guide FACSAria
- QuickStart Guide FACSVantage
- ELISA on AmpliGrid AG480E
Gene expression analysis
RT-qPCR, TaqMan, miRNA, endpoint detection
Endpoint detection
PCR, RTPCR, PAGE & silver staining
- Singleplex or Multiplex PCR
- RT-PCR on total RNA and human single cells
- PAGE and silver staining process instruction
Genetic profiling
AmpFISTR®SEfiler, AmpFISTR®Identifiler, PowerPlex®16, Sequencing
Material Safety Data Sheets
OAX 04207 Sealing Solution (Download pdf / 110 KB)
OAX 04208 Sealing Solution (Download pdf / 110 KB)
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