AmpliSpeed Slide Cycler
- Ultra fast
- Superior homogeneity
- Modular system
- Independent operation
- Automation friendly
- Smallest footprint
Brochures / Flyers
| Download | File / Size | |
|---|---|---|
| AmpliGrid System: Single Cell PCR – Highest sensitivity down to ONE single cell | pdf / 5.79 MB | Start Download |
AmpliGrid Automated Cell Detection | pdf / 462 kb | Start Download |
AmpliGrid AG480E: Activated microliter reaction slide | pdf / 485 kb | Start Download |
Manual
| Download | File / Size | |
|---|---|---|
AmpliSpeed System User Guide | pdf / 2.99 MB | Start Download |
Service Manual AmpliSpeed Instruments | pdf / 115 kb | Start Download |
Application Reports
| Download | File / Size | |
|---|---|---|
Superior sensitivity in RT-PCR of single cells using the AmpliGrid system | pdf / 252 kb | Start Download |
Single cell microRNA assay | pdf / 443 kb | Start Download |
Cell sample storage for gene expression analysis on AmpliGrid AG480F | pdf / 352 kb | Start Download |
Contamination-free ultra low volume pipetting on AmpliGrid AG480F slides | pdf / 370 kb | Start Download |
Getting full profiles from highly redundant low-volume amplifications (LV-PCR) | pdf / 1.47 MB | Start Download |
High sensitivity and cost effective STR profiling on AmpliGrid AG480F using limited sperm material | pdf / 218 kb | Start Download |
Efficient DNA amplification and cycle sequencing from single cells without template preparation | pdf / 378 kb | Start Download |
Novel approach for identifying memory B cells during immune responses | pdf / 3.06 MB | Start Download |
Enhanced DNA amplification from single cells using the Advalytix Cell Extraction Kit | pdf / 198 kb | Start Download |
Case study: Studying the Viral/Bacterial Connection, One Cell at a Time | pdf / 151 kb | Start Download |
Case study: The Evidence Points to Single Cell Analysis for Forensic Applications | pdf / 906 kb | Start Download |
Quantitative RT-PCR from one single cell using the AmpliGrid technology | pdf / 351 kb | Start Download |
Visual control of correct cell placement on AmpliGrid slides prior to molecular analysis | pdf / 164 kb | Start Download |
Identifying virus copies in single cells | pdf / 400 kb | Start Download |
Quantitative multiplex TaqMan® qPCR from single cells using the AmpliGrid platform | pdf / 361 kb | Start Download |
High efficiency one-step lysis and PCR from single bacteria using the AmpliGrid System | pdf / 259 kb | Start Download |
Template amplification and detection of PCR products on AmpliGrid AG480E using microarray technology | pdf / 254 kb | Start Download |
Posters
| Download | File / Size | |
|---|---|---|
Single Circulating Tumour Cell molecular characterization: a feasibility study | pdf / 1.64 MB | Start Download |
Population- and single cell-based strategies to understanding humoral immunity | pdf / 584 kb | Start Download |
AmpliCell and AmpliGrid: A new cell culture and PCR platform for analysis of tumor stem cells | pdf / 3.56 MB | Start Download |
A Reproducible High Throughput Methylome Analysis of a Single Cell | pdf / 818 kb | Start Download |
Zellvergnügen Workflow: Single Cell Molekular Analysis | pdf / 579 kb | Start Download |
Novel approach for identifying memory B cells during immune responses | pdf / 237 kb | Start Download |
Detection of microRNA genes in individual cancer stem cells from breast cancer cell lines using the Advalytix AmpliGrid™ single cell platform and a single cell quantitative RT-PCR method | pdf / 170 kb | Start Download |
Invasionsfreie Pränataldiagnostik auf Einzelzellbasis | pdf / 390 kb | Start Download |
Single cell typing by on-chip Low Volume PCR (LV-PCR) | pdf / 190 kb | Start Download |
Genome-wide expression analysis of single cells using Affymetrix GeneChips | pdf / 1.00 MB | Start Download |
AmpliCell: cell culture-to-PCR based on the AmpliGrid technology | pdf / 595 kb | Start Download |
Scientific Articles
Circulating tumour cells in clinical practice: Methods of detection and possible characterization
Methods (2010), 50(4): pp. 289-297
Single Cell Analysis of Mutations in the APC Gene
Fetal Diagn Ther (2009), 26(3): pp. 148-56
Reduced-volume and low-volume typing of Y-chromosomal SNPs to obtain Finnish Y-chromosomal compound haplotypes
Int J Legal Med (2009), 123(5): pp. 413-418
Preliminary trials of low volume AmpFlSTR® Profiler Plus® amplification using AmpliGrid (AG480F) slides
Forensic Science International (2009), 2(1): pp. 117-118
The combination of single cell micromanipulation with LV-PCR system and its application in forensic science
Forensic Science International (2009), 2(1): pp. 516-517
Automatic retrieval of single microchimeric cells and verification of identity by on-chip multiplex PCR
Journal of cellular and molecular medicine (2009), 10.1111/j.1582-4934.2009.00784.x
Knockdown of transactive response DNA-binding protein (TDP-43) downregulates histone deacetylase 6
EMBO Journal (2009), doi:10.1038, emboj.2009.324
Adult BM generates CD5+ B1 cells containing abundant N-region additions
Eur J Immunol. (2009), 39(9): 2383-94
Single particle adsorbing transfer system
Springer Science + Business Media, Biomed Microdevices (2009) 11: pp. 609–614
Regulation of astrocyte inflammatory responses by the Parkinson’s disease-associated gene DJ-1
FASEB Journal (2009), Vol. 23, article fj.08-125153
Turning to Lipids and Protein
Science Magazine - LIFE SCIENCE TECHNOLOGIES (2009)
Multiplex rt-PCR expression analysis of developmentally important genes in individual mouse preimplantation embryos and blastomeres
Biol Reprod. (2009), 80(1), pp.194-202
Bringing Laser Microdissection and on-chip PCR together
Profiles in DNA (2008)
Single lymphocytes from two healthy individuals with mitochondrial point heteroplasmy are mainly homoplasmic
Int J Legal Med. (2008), 122(3), pp.189-97
Low volume amplification and sequencing of mitochondrial DNA on a chemically structured chip
Int J Legal Med. (2007), 121(1), pp.68-73
Low-volume amplification on chemically structured chips using the PowerPlex16 DNA amplification kit
Int J Legal Med. (2006), 120(1), pp. 42-48
Protocols
- Quantitative RT-PCR on AmpliGrid AG480F
This process instruction describes the quantitative analysis of the housekeeping gene Calm. The reverse transcription is done on the AmpliGrid system using the Qiagen OneStep RT PCR Kit. Afterwards the quantitative amplification is done on a conventional real-time instrument (Stratagene) using SYBR green. (Download pdf / 148 KB) - Singleplex or Multiplex PCR of human DNA on AmpliGrid AG480F
In the singleplex PCR system, one DNA fragment is amplified using human template and the GoTaq® PCR kit or a Qiagen Kit on the AmpliGrid slides.
In the multiplex PCR system, four DNA fragments are amplified using female human DNA template and six DNA fragments.
(Download pdf / 229 KB) - Plasmid Amplification and Sequencing on AmpliGrid AG480F This protocol describes the amplification of plasmid DNA and the following sequencing reaction directly on the AmpliGrid Slide. (Download pdf / 235 KB)
- On-Slide DNA Sequencing using AmpliGrid AG480F
This protocol describes the sequencing procedure after PCR of human DNA directly on the AmpliGrid slide. (Download pdf / 211 KB) - RT-PCR on single mouse cells and extracted RNA using Qiagen reagents and AmpliGrid AG480F This process instruction describes a one-step RT-PCR for mouse B-lymphocytes and extracted total mouse RNA. In this RT-PCR system, b2m and Actin B fragments are amplified using the Qiagen OneStep RT-PCR kit together with AmpliGrid slides. (Download pdf / 249 KB)
- RT-PCR on single mouse cells and extracted RNA using AmpliGrid AG480F This process instruction describes a One-Step RT-PCR for mouse B-lymphocytes and extracted total mouse RNA. In this RT-PCR system, b2m and Actin B fragments are amplified using the Single Cell One-Step RT-PCR kit together with AmpliGrid slides. (Download pdf / 248 KB)
- RT-PCR on human total RNA and single cells using Qiagen reagents and AmpliGrid AG480F This process instruction describes a one-step RT-PCR for human lymphocytes or human total RNA. In this RT-PCR system, b2m and Calm fragments are amplified using the Qiagen OneStep RT-PCR kit together with AmpliGrid slides. (Download pdf / 246 KB)
- RT-PCR on human total RNA and single cells using AmpliGrid AG480F This process instruction describes a One-Step RT-PCR for human lymphocytes or human total RNA. In this RT-PCR system, b2m and Calm fragments are amplified using the Single Cell One-Step RT-PCR kit together with AmpliGrid slides. (Download pdf / 251 KB)
- Genetic Profiling with Applied Biosystems AmpFISTR® SEfiler™ on AmpliGrid AG480F
In this PCR system, 11 specific STR loci are amplified using human DNA as template and the AmpFISTR® SEfiler™ kit from Applied Biosystems together with the AmpliGrid slides. (Download pdf / 238 KB) - Genetic Profiling with Applied Biosystems AmpFISTR® Identifiler® on AmpliGrid AG480F In this PCR system, 15 specific STR loci and the gender-determining Amelogin are co-amplified using human DNA or cells as template and the AmpFISTR® Identifiler® kit from Applied Biosystems together with the AmpliGrid slides. (Download pdf / 235 KB)
- Genetic Profiling with Promega PowerPlex® 16 on AmpliGrid AG480F
This protocol describes the process of amplifying STR DNA fragments using the Promega PowerPlex® 16 System. (Download pdf / 240 KB) - Home-made AmpliGrid adaptor for non-automated P.A.L.M. LCM systems This protocol shows how to assemble a simple and affordable solution to fit AmpliGrid slides into an existing P.A.L.M. LCM system. (Download pdf / 1660 KB)
- TaqMan® microRNA Assay on single cells using AmpliGrid AG480F This process instruction describes an experiment using the TaqMan® microRNA Assay (Applied Biosystems) together with the AmpliGrid slide. (Download pdf / 259 KB)
- ELISA in 1 μL on AmpliGrid AG480E This protocol shows the detection of the target proteins MMP10 and MMP13
on the AmpliGrid AG480E using ELISA technique. (Download pdf / 509 KB) - Polyacrylamide Gel Electrophoresis & Silver Staining This process instruction characterizes the standard processing of a polyacrylamide gel electrophoresis and the consecutive silver staining. This instruction permanently ensures a constant quality of the analysis of PCR products. (Download pdf / 248 KB)
