Make your qPCR more sensitive
You want to perform single cell qPCR but your genes of interest cannot be analyzed because of their low copy number?
Trust the Advalytix AmpliGrid platform to add single cell sensitivity to your qPCR system.
OUR SOLUTION
AmpliGrid integrates with existing workflows for quantitative amplification of genes from just one single cell. We reverse transcribe mRNA directly from cells – no extraction step needed - on the AmpliGrid slide. Then, conduct amplification and quantitative analysis on your existing qPCR system. A simple, sensitive and affordable way to add single cell level investigation to your qPCR investment.
- Our ready-to-use protocols show you how to add single cell sensitivity to your qPCR system for:
- Ambion TaqMan® Gene Expression Cells-to-CT™ Kit
- Brilliant® II SYBR® Green and a Stratagene Mx3005P® - Attend our Single Cell qPCR Workshop in cooperation with BioEPS/TATAA Biocenter Germany (www.bioeps.com).
- Learn more: single cell handling feature on www.gene-quantification.info.
USER STORIES
- Scientists from the Johannes Gutenberg University Mainz, Germany use AmpliGrid to study individual preimplantation embryos and blastomeres
- Karin Görner and Katharina Pachmann from the Friedrich Schiller-University, Jena, Germany use AmpliCell and AmpliGrid as a new cell culture and PCR platform to analyse gene expression in tumor stem cells using qPCR.
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