Single Cell One-Step RT-PCR System
Pre-optimized reagents for single cell and 1 μL RT-PCR
- Pre-optimized for performance and compatibility with AmpliGrid single cell and 1 μL RT-PCR
- One-Step RT-PCR setup
- Master mix format to minimize hands-on time
- Optional on-slide DNase treatment or cell lysis with AmpliGrid Cell Extraction Kit for hard to lyse cells
|
Component |
Amount |
| 2x Single Cell RT Reaction Buffer | 250 µl |
| Single Cell RT enzyme mix | 20 µl |
| 5x Single Cell RT enhancer | 60 µl |
| RNase Inhibitor (10 U/μL) | 10 µl |
| DNase I Reaction Buffer | 40 µl |
| Nuclease free water (DEPC treated) | 1000 µl |
Contents of kit are sufficient to run 5 AmpliGrid AG480 slides (240 x 1 µl reactions)
