Single cell molecular analysis should not be cumbersome, unreliable and expensive but Simple, Sensitive, and Affordable^®, so YOU can focus on science. SIMPLE: Whether you capture single cells via flow sorting, micromanipulation, or laser capture microscopy, or whether your molecular analysis involves qPCR, PAGE, CE, or sequencing, our workflows integrate Advalytix and third party equipment. SENSITIVE: Whether you need PCR, rtPCR, qPCR, or sequencing, amplifying in AmpliGrid slides rather than tubes or MTPs improves amplification sensitivity. Click for sensitivity evidence: PCR , rtPCR , qPCR , sequencing. AFFORDABLE: Our AmpliSpeed cycler is priced competitively with standard thermocyclers. Our pricing plans for AmpliGrid slides, combined with reagent saving 1µl assays and high success rates reduce consumable spending.
SINGLE CELL WORKFLOW: view full movie, or view Step by Step below Step 1: Isolation & deposition
	Three easy ways to isolate and deposit single cells on AmpliGrid slides^{see platform overview video}: Flow Cytometry ^{see video} • For liquid samples with cells in suspension • High throughput cell sorting/deposition • Placement accuracy superior to sorting into MTPs Laser Capture Microdissection (LCM) • Isolate cells from tissue • Direct deposit of cells with PALM system Micromanipulation ^{see video} • For standard workflows using capillaries for cell picking and transfer, e.g. IVF • Automation available
Step 2: Quality control ^{see video}
	Take the guess work out of cell placement! Stain your cells (e.g. fluorescent DNA stain) prior to deposition. Then check sorted slide for accurate cell placement with a fluorescent microscope. This process can also be automated. Visual QC result: verify correct cell placement
Step 3: Amplification ^{see video}
	AmpliSpeed Slide Cycler for AmpliGrid slide For amplification, simply add 1µL of mastermix and cover with 5µL of cover oil. Hydrophobic areas confine mastermix and oil to their designated areas – no contamination, no mess. • Sensitive amplification of DNA, mRNA, mtDNA • Enhanced sensitivity with DNA extraction kit • Manual or automated pipetting (see video on Youtube) • 1uL assay volume • Pre-amplification for qPCR
Step 4: Analysis ^{see video}
	Gel Analysis/ Page Determine presence/absence of up to 5 genes/transcripts, or isolate fragments for subsequent sequencing. Let us design your multiplex assay. Capillary Electrophoresis Detect up to 25 genes/ transcripts from a multiplex assay. Get semi-quantitative results by mapping the area under the peaks into low, medium and high expressers. Sequencing Combine RT-PCR/PCR with sequencing on AmpliGrid qPCR Add AmpliGrid’s convenient single cell workflow and sensitivity with to established qPCR data analysis.