It is well-known that individual microorganisms can differ widely from each other in terms of their genetic composition, physiology, biochemistry, and behaviour. Apart from being of extraordinary relevance for basic scientists this genetic and phenotypic heterogeneity has also important consequences for a number of economical interests including the productivity and stability of industrial fermentations, the efficacy of food preservatives, and the production of antibiotics and biocides.
Single cells analysis can be considered as key method for microbiologists giving new insights into otherwise invisible processes such as interspecies gene transfer and chemical communication, physicochemical microbe-surface and host-pathogen interactions, mechanical properties of individual cells like turgor pressure and elasticity as well as microphysiological processes including metabolite, protein, or elemental localization.

With the AmpliGrid system Olympus has developed innovative technology and Simple, Seamless, and Sensitive® workflows for genetic analysis of single eukyotic cells. The AmpliGrid system represents a superior amplification platform for ultra low volume applications in the 1µL range, based on a chemically structured microscope slide. With this innovative system, sensitivity, efficiency and reproducibility are reliable constants for single cell analysis. We offer detailed protocols and technical support to help you port your current analysis method into the of 1µL world AmpliGrid.