Polar Body / Single Cell Analysis Kit
- Polar Body Analysis
- Detection of presence or absence of all human chromosomes
- Minimised sample loss
- Minimal initial outlay
Aberrations in the number of chromosome copies are major causes of embryo loss, both before and after implantation. It has been shown that those aneuplodies are responsible for the failure of embryo implantation as well as the occurrence of first trimester miscarriages. It has been shown that aneuplodies are often responsible for the failure of implantation of embryos and also for increasing the occurrence of miscarriages in the first trimester of a pregnancy. The incidences of aneuplodies increase with the age of the mother such that 30–50% of the oocytes of a 35 year old healthy woman show aneuplodies.
With polar body analysis it is possible to detect irregularities in the chromosome arrangement during an IVF cycle. More importantly, polar body analysis is the only legally accepted method for chromosomal examination of unfertilised oocytes.
Advalytix AG now offers a superior nucleic acid-based screening test for the analysis of the presence or absence of all human chromosomal genetic material from a single cell.
We provide an easy to use platform, based on a microscope slide, for single cell deposition. Any standard equipment for single cell manipulation e.g., micromanipulators or FACS are compatible with our slides. Continuous documentation of the process and the 100% safety presence of one single cell are assured on this high-quality optical substrate.
Samples are analysed using a redundant two step multiplex PCR method on each chromosome. Complex primer design guarantees reliable results combined with an easy read out based on polyacrylamide gel electrophoresis (PAGE).
Due to the direct amplification of the sample material the loss of chromosomes or, in the worst case scenario, of the whole cell during downstream processing is minimised.
