The analysis of single cells using quantitative PCR often leads to unsatisfactory results due to the low gene copy number contained in a single cell sample.  In addition, conventional single cell workflows are cumbersome, error-prone, and make inefficient use of precious template material.
In order to combine the easy-to-use workflows and single cell sensitivity of the AmpliGrid platform with quantitative PCR results, we developed a qPCR pre-amplification protocol.  This protocol directly pre-amplifies single cells using the AmpliGrid 1µL PCR platform – no separate extraction step needed. The pre-amplified amplicons are then transferred onto a standard qPCR system for qPCR analysis.

Illustrating this qPCR protocol, our application report describes how to measure the mRNA expression of two genes in single human lymphocytes.  This report was featured in the May email newsletter of www.gene-quantification.info/.

For further information on single cell qPCR, also visit the single cell qPCR feature  and our company feature on the Gene-Quantification qPCR portal site or meet us here.

Please come back to check here for updates on our single cell qPCR activities.  We will shortly announce a single cell qPCR seminar in Munich, publish an interview with one of our single cell qPCR customers, and are working on a multiplex single cell qPCR protocol.  For further updates, please subscribe to our newsletter.