Single Cell Analysis Kit
- Polar Body Analysis
- Detection of presence or absence of all human chromosomes
- Minimised sample loss
- Minimal initial outlay
Overview Polar Body Analysis
Polar bodies are cell structures found inbetween oocyte and zona pelucida. They are generated by asymmetrical meiotic cell division during oogenesis. As an egg matures, it goes through a two-step division process, dividing once at the time when ovulation would occur and again at the time of fertilization. The polar bodies are the by-products of this division, and are essentially being discarded by the egg, consequently they are relatively small and contain little cytoplasm. Since there are two divisions, there are usually two polar bodies inside the ovum, one diploid and one haploid. By analyzing the polar bodies, it is possible to infer the genetic status of the egg. Therefore, polar body analysis allows us to test the mother’s genetic contribution to the embryo.
The reduction in number of the chromosomes in the process of maturation of the ovum.
Polar bodies can be taken out without any negative interference on the egg cell. Current methods such as fluorescent in situ hybridisation (FISH), only usually cover a panel of 5–10 chromosomes, whereas aberrations in any of the chromosomes can lead to embryo loss. The Advalytix Single Cell Analysis Kit covers the full panel of genetic content of all human chromosomes. This offers advanced analysis which is especially useful for patients with repeated in vitro fertilisation failures or repeated miscarriages.
Location of polar bodies in the oocyte (p.gl.)
